quantitative
iHC
Automated IMMUNOHISTOCHEMISTRY (IHC) staining driven by over 250 optimized protocols, new antibody optimization, quantitative data, and a customer service we are proud of.
Immunohistochemistry Powered By
DATA
the process
Tissue
Staining
Imaging
Analysis
THE REVEAL ADVANTAGE
Over 250 IHC Protocols
Over 250 optimized IHC and IF protocols to suit the needs of any project. If you don’t find what you need, our team of experts can optimize new antibodies on request.
Antibody Optimization
Offering new antibody optimization to suit the unique needs of each individual study.
Imaging & Data
imageDx generates quantitative IHC data from tissue such as percent positivity, stain intensity, positive cells per mm2, automated tumor/stroma detection, and more.
Quality Management
Quality is at the heart of everything we do. Our Quality Management program impacts every stage of our workflow to ensure the best results.
Expert Team
Trust our experienced team who are passionate about quality, meaningful data, and great customer service.
Great Customer Service
We operate as partners in your extended team. Our friendly and knowledgeable staff work hard to build long-term relationships that have a positive impact.
DATA POWERED research
Trust our team of experienced scientists to take your samples from fixed tissue to stained slides, high resolution whole slide images, and quantitative data.
Select IHC Applications
IMMUNO-ONCOLOGY
Optimized quantitative IHC protocols for a wide range of immuno-oncology markers including: CD3, CD4, CD8, CD61, FoxP3, PD1, PDL1, CD45, CD11b, CD11c, CD20, CD103, CD14, CD163, CD19, CD206, CD68 and more…
Multiplex immunofluorescence IO panels also available.
NEUROSCIENCE
Optimized quantitative IHC protocols for a wide range of neuroscience markers including:
GFAP, NeuN, Iba1, S100, NFM
Multiplex immunofluorescence neuro panels also available.
Antibody optimization
BRONZE - single antibody
A single antibody, selected by the client or our technical team, is tested on positive control tissue at four different concentrations, each with two different antigen retrieval conditions plus appropriate negative controls.
SILVER - multiple antibodies
A number of antibodies (usually 3 – 4) are selected from different sources that target the same biomarker. These antibodies are each tested and compared to determine which gives the best results for the tissue(s) of interest.
GOLD - clinical validation
This level of optimization is most suited for antibodies that will be used in clinical applications such as companion diagnostics. Antibodies are tested and optimized as described above, with the addition of a full clinical validation following guidelines from the College of American Pathologists (CAP).
