NASH: IHC Digital Assays for Immune Cell Quantification

Research into developing effective treatments for non-alcoholic steatohepatitis (NASH) is becoming more important as the incidence of NASH increases in the general population. Mouse and rat models are used in the development of NASH therapeutics to help investigators better understand the pathophysiological mechanisms of the disease. Treatment strategies for NASH that target and modulate the immune response require a deeper understanding of the immune cells present in NASH liver tissue during disease continuum. 

Reveal offers optimized immunohistochemistry (IHC) protocols with validated antibodies to identify specific populations of immune cells within formalin fixed, paraffin-embedded (FFPE) liver tissue. Application of a panel of these markers can identify resident immune cells within the disease milieu as well as better understand changes in the immune cell populations post-treatment.  A list of the most popular optimized immune markers can be found below:

CD3e staining of mouse FFPE NASH liver tissue showing scattered CD3e-positive T cells throughout the tissue.

Our scientists have extensive experience optimizing protocols for novel and specific markers for T cell and macrophage subsets unique to the requirements of your project.  Contact us to talk and learn more.

Data Powered Pathology

Quantitative cell-by-cell data generated from across each entire tissue section is a powerful tool to assist in understanding the immune cell population associated with NASH.  imageDx: IHC Digital Assays have been optimized to generate immune cell data specific for each antibody.  Typical reporting includes percent positivity, stain intensity, positive cell count, total cell count, tissue area, and more to help researchers gain deeper insights into the immune response.  Data to assess the ratio of multiple markers can also be provided, giving deeper insight into the immune cell population.  

Automated NAS and Fibrosis Scoring

imageDx: NASH provides quantitative data from H&E and Masson’s Trichrome or Picrosirius Red stained slides to increase the accuracy, reproducibility, and scale of NASH and fibrosis analyses.  This deep learning-based digital assay precisely quantifies inflammation in human or mouse liver tissue, along with steatosis (microvesicular and macrovesicular), hepatocellular ballooning, and fibrosis.

Contact us to apply the power of Digital Assays to your NASH research.

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