imageDx PRism

AI-powered multiplex immunofluorescence data and CellCarta expertise for Akoya® & Ultivue®

Spatial biology is a powerful approach to patient stratification and therapy guidance in the field of immuno-oncology. imageDx PRISM from Reveal Biosciences applies the latest advances in AI to novel pattern discovery and spatial biomarker characterization, revealing new patient data insights.

SELECT YOUR MULTIPLEX DATA OUTPUTS

Three tiers of multiplex immunofluorescence DATA:

Tier I: Quantitative cell-by-cell data
Tier II: Microenvironment & spatial data analysis
Tier III: AI pattern discovery & predictive models

Multiplex Immunofluorescence Data Outputs

THREE AI multiPLEX OPTIONS

Quantitative Cell-by-Cell Data

Quantitative Cell-by-Cell Multiplex Immunofluorescence Data

Quantitative cell-by-cell data reported across the entire image including percent positivity & colocalization.

Microenvironment & Spatial Data Analysis

Spatial Data Analysis for Multiplex Immunofluorescence

Multi-cell microenvironment & spatial data analysis includes cluster analysis, proximity data for predefined markers, and distance to tumor-stroma interface.

AI Pattern Discovery & Predictive Models

AI Pattern Discovery for Multiplex Immunofluorescence Data

Custom AI models developed using graph neural networks to interrogate pattern formation and/or predict patient outcomes.

CellCarta Multiplex Immunofluorescence Expertise

Multiplex immunofluorescence is performed at the CellCarta specialized CAP/CLIA laboratory at Lake Forest, CA (formerly Mosaic Laboratories)

Akoya Opal® Chemistry

Motif PD-1/PD-L1 Panel – CD8+CD68+FoxP3+PD-1+PD-L1+ PanCK

Other Available Panels Include – GrB+NKp46+IFNg+CD8GrB+PanCK+CD3+Ki67+CD8

Custom Assay Development Available

Akoya Opal Chemistry Multiplex Immunofluorescence Powered by CellCarta

Ultivue® multiplex Panels

FixVUE panels – Choose from pre-configured 4- and 8-plex panels:

  • Immuno8 panel – CD3+CD4+CD8+CD68+FoxP3+PD-1+PD-L1+CK/SOX10+Nuclear counterstain
  • PD-L1 panel – CD8+CD68+PD-L1+PanCK/SOX10+Nuclear counterstain
  • T-reg panel – CD4+CD8+ FoxP3+PanCK/SOX10+Nuclear counterstain
  • MDSC panel – CD11b+CD14+CD15+HLA-DR+Nuclear counterstain
  • PD-1 panel – CD3+CD45RO+PD-1+PanCK/SOX10+Nuclear counterstain
  • T-act panel – CD3+Granzyme B+Ki67+PanCK/SOX10+Nuclear counterstain
  • APC panel – CD11c+CD20+CD68/CD163+MHC Class II+Nuclear counterstain

FlexVUE panel – Start with the FixVUE Immuno8 panel (above) and substitute up to two markers from

  • Ki67, GrzB, CD45RO, HLA-DR, CD56, CD20, CD11C, CD163, CD206, CD14, CD15, CD11b, MHCII

U-VUE panel – Customize your 8-plex panel from a list of Ultivue marker options.

Ultivue FixVUE, FlexVUE, and U-Vue Mutiplex Immunofluorescence

Advanced Multiplex Immunofluorescence Powered By:

Contact Us

Reach out to our friendly team

Learn how data powered pathology can enhance your research, clinical trial, or pathology workflow.

Pathology Revealed

Sign up to receive updates on the latest AI-powered pathology breakthroughs, access digital pathology resources, and more.