imageDx PRism
AI-powered multiplex immunofluorescence data and CellCarta expertise for Akoya® & Ultivue®
Spatial biology is a powerful approach to patient stratification and therapy guidance in the field of immuno-oncology. imageDx PRISM from Reveal Biosciences applies the latest advances in AI to novel pattern discovery and spatial biomarker characterization, revealing new patient data insights.
SELECT YOUR MULTIPLEX DATA OUTPUTS
Three tiers of multiplex immunofluorescence DATA:
Tier I: Quantitative cell-by-cell data
Tier II: Microenvironment & spatial data analysis
Tier III: AI pattern discovery & predictive models
THREE AI multiPLEX OPTIONS
Quantitative Cell-by-Cell Data
Quantitative cell-by-cell data reported across the entire image including percent positivity & colocalization.
Microenvironment & Spatial Data Analysis
Multi-cell microenvironment & spatial data analysis includes cluster analysis, proximity data for predefined markers, and distance to tumor-stroma interface.
AI Pattern Discovery & Predictive Models
Custom AI models developed using graph neural networks to interrogate pattern formation and/or predict patient outcomes.
CellCarta Multiplex Immunofluorescence Expertise
Multiplex immunofluorescence is performed at the CellCarta specialized CAP/CLIA laboratory at Lake Forest, CA (formerly Mosaic Laboratories)
Akoya Opal® Chemistry
Motif PD-1/PD-L1 Panel – CD8+CD68+FoxP3+PD-1+PD-L1+ PanCK
Other Available Panels Include – GrB+NKp46+IFNg+CD8 & GrB+PanCK+CD3+Ki67+CD8
Custom Assay Development Available
Ultivue® multiplex Panels
FixVUE™ panels – Choose from pre-configured 4- and 8-plex panels:
- Immuno8™ panel – CD3+CD4+CD8+CD68+FoxP3+PD-1+PD-L1+CK/SOX10+Nuclear counterstain
- PD-L1 panel – CD8+CD68+PD-L1+PanCK/SOX10+Nuclear counterstain
- T-reg panel – CD4+CD8+ FoxP3+PanCK/SOX10+Nuclear counterstain
- MDSC panel – CD11b+CD14+CD15+HLA-DR+Nuclear counterstain
- PD-1 panel – CD3+CD45RO+PD-1+PanCK/SOX10+Nuclear counterstain
- T-act panel – CD3+Granzyme B+Ki67+PanCK/SOX10+Nuclear counterstain
- APC panel – CD11c+CD20+CD68/CD163+MHC Class II+Nuclear counterstain
FlexVUE™ panel – Start with the FixVUE™ Immuno8™ panel (above) and substitute up to two markers from:
- Ki67, GrzB, CD45RO, HLA-DR, CD56, CD20, CD11C, CD163, CD206, CD14, CD15, CD11b, MHCII
U-VUE™ panel – Customize your 8-plex panel from a list of Ultivue marker options.
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